The first step involves the preparation of a nutrient solution. This is done by dissolving 10g of sucrose as well as 10mg of boric acid in 100ml of water.
Pour a few drops of this solution onto the cavity slide. Then, use a brush or fingers to gently dust a few pollen grains from the stamen of mature flowers.
Let the slide set for 5 mins. Then, use the microscope to view the slides in 30-minute intervals.