Take young buds of Tradescantia or immature male spikelet‟s of Bajra (Millet) and fix them in freshly prepared Carnoy fixative for 10 hrs. After rehydrating in 90% and 70% alcohol store the material in 70% alcohol.
Take a bud of Tradescantia or few smaller anthers from the male spikelet of Bajra in 70% alcohol in a watch glass.
Dissect out the bud or dissect each anther taken from the spikelet with needles & keep them in 45% acetic acid.
Put few anthers on the slide and few drops of Acetocarmine stain over them. Warm the slide gently a few times, but never let it boil.
Drain off excess stain and put few drops of 45% acetic acid.
Now put cover slip over the material and cover it with filter paper or blotting paper. Press the cover slip with thumb so as to rupture the anthers.
Observe the slide under the microscope first in low power and then in high power.