Procedure

Take an onion and remove its outermost peel.

Now cut a small part from an inner scale leaf with the help of a blade.

Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.

Keep this peel in a watch glass containing water?

Add two drops of safranin stain in the watch glass to stain the peel.

Take a clean slide and put a drop of glycerin in the center of the slide.

With the help of a brush and needle transfer the peel on the slide. Glycerin prevents the peel from drying up.

Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.

Remove any excessive glycerin with a filter paper.

Observe the prepared mount of the peel under the low and high magnification of a compound microscope.